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Objective To explore the value of the combined detection of serum CEA ,CA199 and CRP in the diagnosis and prog‐nosis of colorectal cancer .Methods Totally 150 patients with colorectal cancer treated in our hospital during 2012‐2014 were se‐lected as the observation subjects ,including 97 cases of histopathologically diagnosed of colorectal cancer without metastasis verified by the assisted examinations as the non‐metastatic group and 53 cases of metastasis verified by the assisted examinations as the me‐tastatic group ;at the sametime ,80 individuals undergoing the physical examination were taken as the control group .Then the levels of serum CEA ,CA199 and whole blood CRP level before surgery and after operation combined with 4 courses of chemotherapy or radiotherapy were compared between the observation group and the control group ,and between the non‐metastatic group and meta‐static group .Results The levels of serum CEA ,CA199 and whole blood CRP before treatment in the observation group were sig‐nificantly higher than those in the control group ,there was statistically significant difference between two groups (P<0 .01) ,more‐over the level of each indicator in the metastatic group of the observation group was higher than that in the non‐metastatic group , the difference between two groups was statistically significant (P<0 .01);compared before treatment ,the levels of serum CEA , CA199 and whole blood CRP after treatment in the observation group were decreased ,the differences between two groups were sta‐tistically significant (P<0 .01);moreover the sensitivity of the combined detection of CEA ,CA199 ,CRP was significantly higher than that of single indicator detection ,the difference was statistically significant (P<0 .01) .Conclusion The combined detection of serum CEA ,CA199 and whole blood CRP is of great importance for the diagnosis and prognosis of colorectal cancer ,which is con‐ducive to the early diagnosis and prognosis evaluation of colorectal cancer .
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Objective To investigate the effect of gene therapy which was mediated by electroporation on the expression of basic fibroblast growth factor (bFGF) duning mandible distraction.Methods Bilateral mandibular osteotomies were performed in New-Zealand rabbit.After a latency of 3 days,the mandibles were elongated using distractors with a rate of 0.8 mm/d for 7 days.The rabbits were randomly divided into 5 groups:2 μtg recombinant plasmids pIRES-hVEGF165-hBMP2,pIRES-hBMP2,pIRES-hVEGF165,pIRES and normal saline (NS) were injected into the distraction area of groups A,B,C,D and E,after completion of distraction,respectively.The lengthened mandibles were harvested and processed for immunohistochemical detection of bFGF,and the mean optic densities and integral optical density of bFGF positive cells were measured by computerized image analyzer.Results bFGF mainly located in fibroblasts,giant monocytes,polynuclear phagocytes,osetocytes,and osetoblasts in the connective tissue around bone tissue.The strongest expression was observed at the 7th day,and weakened at 14th day of consolidation stage,there were no significant difference among groups A,B and C,at the 7th day of consolidation.However,there were significant differences between gene therapy groups (A,B and C) and control groups (D and E) (P＜0.01).Conclusions Gene therapy can enhance and prolong the expression of bFGF in distraction gap,which promotes the cell differentiation and proliferation,extracellular matrix synthesis and new bone formation during distraction osteogenesis.This is probably one of the molecular mechanisms of the gene therapy promoting new bone formation in distraction gap.
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Objective To iavestigate the influence of two disinfectants methods on disintection efficiency and model precision of dental impression.Methods Alginate impressions,alginate and agar complex impressions and silicon rubber impressions were made according to a unified standard.Chlorinous disinfectant solution and acidic oxidative potential water were used to disinfect the infected impressions.The influence of two disinfectants on disinfection efficiency and model precision of dental impression was compared.Results Two disinfectants for 30 minutes had significant disinfection effect on these three kinds of impressions.The effective rate reached 99.48％.Chlorinous disinfectant solution bad no significant influence on the model precision of alginate impressions and silicon rubber impressions,but had significant influence on the model precision of alginate and agar complex impressions.Acidic oxidative potential water did not influence the model precision of the three dental impressions.Conclusions Acidic oxidative potential water can be used to disinfect the three dental impressions.Chlorinous disinfectant solution can be used to disinfect alginate impressions and silicon rubber impressions,but cannot be used to disinfect agar complex impressions.
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Objective To investigate the expression patterns of major bone growth factors and bone morphogenetic protein-2 (BMP2) in the distracted calluses following transfected gene during mandibular distraction osteogenesis in a rabbit model.Methods Bilateral mandibular osteotomies were performed in New-Zeland rabbits.After a latency of 3 days,the mandibles were elongated using distractors for 7 days.After the completion of distraction,the rabbits were randomly divided into 5 groups.Three animals each were sacrificed at the end of the delay phase,at 7,14,and 28 days after completion of distraction,respectively.The lengthened mandibles were harvested and processed for immunohistochemical detection of BMP2 expression,the mean optic densities and integral optical density of BMP2 positive cells were measured by computerized image analyzer.Results Elevated cellular expression of BMP2,in the distraction gap,was observed following mandibular distraction.BMP2 staining was mainly located in inflammatory cells,and the connective tissues arrounding the new bone.Their strongest expression was the 7th day,some of those growth factors expressed weakly or negatively.Conclusions Electroporation-mediated gene transfection can promote BMP2 expression effectively,which plays an important role in cell differentiation and proliferation during distraction osteo genesis.The BMP2 stimulates extracellular matrix synthesis,induces the proliferation and differentiation of fibroblasts and osteoblasts,which then promotes the new bone formation and repair.
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Objective To study the chemical constituents of Paeonia veitchii. Methods Isolation and purification were carried out on repeated silica gel column chromatography. The structures of the compounds were identified by physico-chemical properties and spectral analyses. Results Nine compounds were isolated and identified as paeoniflorin (Ⅰ), hydroxypaeoniflorin (Ⅱ), benzoylpaeoniflorin (Ⅲ), benzoylhydroxypaeoniflorin (Ⅳ), albiflorin (Ⅴ), paeonisothujone (Ⅵ), mudanpinoic acid A (Ⅶ), 2-hydroxybenzyl alcohol (Ⅷ), bis (2-hydroxybenzyl) ether (Ⅸ). Conclusion Compound Ⅸ is obtained from natural products for the first time. Compounds Ⅳ, Ⅵ-Ⅸ are isolated from this plant for the first time.
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Yunnan Strain Information System has been developed by using the Programming Lan guage and Database Engine It includes information of over 10,000 strains that are stored in Yunnan University Institute of Microbiology Strain Library The S ystem makes a convenience for management of Strain Library and supply i mportant information for study these